Partial Purification of Catalase Enzyme from Muscle Tissue of Dusky Spinefoot (Siganus luridus)
نویسندگان
چکیده
Catalase, one of the antioxidant enzymes, decomposes hydrogen peroxide into water and oxygen. From discovery catalase, several studies have been carried out to reveal its importance in health, food cosmetics industries etc., these are still ongoing. In this study, catalase enzyme was partially purified from muscle tissue Dusky spinefoot (Siganus luridus). Purification procedure consisted homogenate preparation, ammonium sulfate precipitation dialysis. The precipitated range 40-60 % Ammonium Sulfate concentration. optimum buffer determined as 200 mM phosphate buffer, pH 8.0 substrate concentration 24mM for H₂ O₂, respectively.
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ژورنال
عنوان ژورنال: Black sea journal of agriculture
سال: 2023
ISSN: ['2618-6578']
DOI: https://doi.org/10.47115/bsagriculture.1213728